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jove.com
Video: DNA Ligation Reactions: Principle, Procedure, and Applications
197.3K Views. In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular ...
197.5K views
Feb 1, 2013
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