To analyze splicing activity, they selected HNRNPH1 pre-mRNA (heterogeneous nuclear ribonucleoprotein H1) including a 56-nt short intron.

Finally, biochemical analyses and splicing assays with various mutant SPF45 proteins helped establish the model of SPF45-dependent splicing on a short intron with a truncated PPT (Figure 1).

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They found a much greater number of aberrant intron 3’ splice sites than were seen in cells with wild-type SF3B1 (Wang, L. et al. Cancer Cell 2016;30:750-763).

Group II intron RNAs self-splice in vitro but only at high salt and/or Mg²⁺ concentrations and have been thought to require proteins to stabilize their active structure for efficient splicing in vivo.

The strongest splicing repression in HNRNPH1 pre-mRNA with 56-nt intron was caused by knockdown of SPF45, but no splicing repression was observed in pre-mRNA with control 366-nt intron.

"Our work provides exciting new insights into the mechanism of minor intron recognition and sheds light on the evolution of the splicing machinery. It opens up new possibilities for studying other ...

The authors show that after the spliceosome is finished splicing the mRNA, it remains active and can engage in further reactions with the removed introns. This discovery provides the strongest ...

Alternative splicing promotes adaptation to environmental changes; in fungi, the most common example of alternative splicing is intron retention. To explore the relationship between splicing and ...