This gives the primers an opportunity to bind, or anneal, to their complementary sequences on the single strands of DNA (Figure 3).

The various components required for PCR include a DNA sample, DNA primers, free nucleotides called ddNTPs, and DNA polymerase.

Primers – A primer is a short strand of nucleotides that will bind to the 3’ end of the template DNA strand allowing DNA polymerase to add free DNA nucleotides.

Sharyn A. Rusk, Frederick W. Spiegel, Steven B. Lee, Design of Polymerase Chain Reaction Primers for Amplifying Nuclear Ribosomal DNA from Slime Molds, Mycologia, Vol ...

A DNA polymerase purified from extracts of chick embryo catalyzes the synthesis of poly (dT) in the presence of poly (rA) or poly (dA) as template and poly (dT) or oligo (dT) as primer. Oligo- or ...