Post this CleanScribe RNA Polymerase is a novel DNA-dependent RNA polymerase that catalyzes the in vitro transcription (IVT) of a recombinant gene regulated by the T7 promoter.

Greedy accumulation-directed evolution generated multi-site combinatorial mutants of T7 RNA polymerase (T7 RNAP), breaking through the thermal stability barrier.

Rather than tackling the entire protein synthesis machinery, the authors zoned in on one critical member: the T7 RNA polymerase. The darling of the synthetic biology sphere, this enzyme is a hefty ...

It incorporates commercially available cap analogs more efficiently than wild-type T7 (WT T7) RNA polymerase, generates less undesirable double-stranded RNA (dsRNA) byproduct and provides ...

Initial transcription by RNA polymerase proceeds through a "DNA scrunching" mechanism, in which the enzyme remains stationary on promoter DNA and pulls into itself downstream DNA. [Proposed ...

How chromatin responds to transcription-blocking DNA lesions is unclear. This study reveals that RNA polymerase II (RNAPII) ...

CGGBP1 binds to short CGG repeats in gene promoters, recruiting RNA:DNA helicases like DDX41 to ensure proper transcription ...