Kathleen Danna and Daniel Nathans obtained the restriction endonuclease map by studying the specific cleavage of SV40 DNA by Haemophilus influenzae restriction enzymes.

The use of restriction enzymes to characterize DNA has been popular since the 1970s. Today, this technique remains one of the easiest and fastest ways to evaluate DNA sequences. As with most ...

The 'recombination' potential of restriction enzymes was first demonstrated by Janet Mertz and Ronald Davis. They showed that the R1 restriction endonuclease produces 'staggered' breaks ...

Enzymes called restriction endonucleases sever DNA at certain recognition sequences. Restrictions endonuclease products are the DNA fragments that are produced.

FokI is a unique restriction enzyme that identifies a certain DNA sequence and cleaves 9–13 base pairs (bp) away from it non-specifically.

Plastid DNA was isolated from the chloroplasts of tomato (Lycopersicon esculentum var Traveler 76) leaves and the chromoplasts of ripe tomato fruit. Comparisons of the two DNAs were made by ...

The active form of the restriction enzyme is a dimer, as determined by gel filtration. Constructed transformants of P. stuartii 164 that carry the Pst I system inserted into pBR322 produce ...

“Most familiar restriction enzymes are in a family called Type II RM systems—and as far as anti-phage weapons go, they’re quite rudimentary.” continues Stoddard. “Many of these systems work by ...

Cloning Design Made Simple Uncovering a viable solution for the assembly of one or multiple fragments into a vector using the seamless type IIS restriction endonuclease approach is not trivial as ...