Studies of gene expression in plants and other organisms rely on the extraction of high-quality RNA. Although numerous protocols for RNA extraction have been developed, most of these are plant ...

Researchers at the University of California, Berkeley, have developed a new protocol for RNA extraction that can be used across land plants, which comprise over 300,000 species.

The mouse bEnd.3 cell RNA samples were derived from 3.1–400 × 10 3 cells and isolated with nucleic acid extraction filter plates, using either a commercial reagent protocol (blue squares) or ...

RNA extraction is a notoriously tricky and sensitive lab procedure, but new protocols out of the University of Florida are quicker, more effective, and more reliable than previous methods. The ...

Scientists have developed a new extraction protocol for RNA-seq and metabolomic analysis, offering a more complete picture of cellular activity than either technique on its own. The protocol ...

Reference: Madaj ZB, Dahabieh MS, Kamalumpundi V, et al. Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-‘omics analysis of the liver metabolic response to ...

If only DNA and RNA are required then the protocol can be terminated at this point. Protein Isolation The flow-through retained from the RNA binding column now contains just the protein fraction.

An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in ...

Protocol Published: 27 June 2006 Isolation of specific RNA-binding proteins using the streptomycin-binding RNA aptamer Nikolai Windbichler & Renée Schroeder Nature Protocols 1, 637–640 (2006 ...

This protocol enables simultaneous analysis of host and bacterial transcripts by RNA-seq. It includes procedures for efficient host and bacterial cell lysis, barcoding of samples and analysis of ...

Researchers extract RNA and DNA from biological samples as a starting point for various downstream biomolecular applications, such as next-generation sequencing, PCR, and microarray analysis. The ...