Scientists have developed a new extraction protocol for RNA-seq and metabolomic analysis, offering a more complete picture of cellular activity than either technique on its own. The protocol ...

COLD SPRING HARBOR, N.Y. (Thurs., Oct. 1, 2009) – The study of RNA has long been the tool of choice for understanding where and when genes are expressed in a cell, tissue, or organism during ...

An optimized method for RNA extraction from highly invasive glioma cells embedded into Matrigel was achieved by using TRIzol, used for matrix dissociation, cell lysis and RNA separation, in ...

For instance, an optimised phenol–chloroform RNA extraction protocol has been developed to reduce contaminants, notably residual phenol and salts, thereby enhancing the accuracy of RNA ...

Researchers extract RNA and DNA from biological samples as a starting point for various downstream biomolecular applications, such as next-generation sequencing, PCR, and microarray analysis. The ...

The secondary metabolite content in mangrove roots can interfere with RNA extraction, making it unsuitable for downstream applications. In response, scientists from the Central University of Kerala ...

Extracted RNA was prepared for sequencing using the ARTIC nCoV-2019 sequencing protocol and sequenced on the ONT Mk1C sequencing platform, followed by data analysis. Additionally, real-time RT-PCR ...