Objective: To present an optimized method of protein extraction from male breast FFPE samples for proteomic analysis. Methods: Four protein extraction protocols were optimized and tested in female ...

Scientists can choose from many protein purification protocols, but some methods fail to produce sufficient sample purity. By selecting the right chromatography method, researchers can optimize the ...

In the protocol in our lab at the German Cancer Research Center (DKFZ), the affinity tag is Staphylococcus aureus protein A, which can be purified on immunoglobulins as the affinity matrix.

This VFD purification protocol is compatible with bead- or membrane-based purification systems and with both clarified and non-clarified lysates, the team notes.

The standard ÄKTAxpress system, which can purify as much as 50 mg of target protein, contains four modules, each of which houses a pump, air sensor, UV detector, and integrated 96-well plate fraction ...

New RNA-seq, metabolomics protocol offers more efficient extraction that maintains data integrity Date: May 2, 2023 Source: Van Andel Research Institute Summary: Scientists have developed a new ...

Efficient and affordable protein purification is essential for developing next-generation drugs and vaccines. As biopharmaceuticals such as bispecific antibodies and cell and gene therapies continue ...