Enhancing productivity with the Thermo Scientific™ KingFisher™ PlasmidPro Maxi Processor—a complete walk-away maxi-scale plasmid purification Introducing automated high-throughput Applied Biosystems™ ...

In the Laboratory Published: August 1999 Purification of plasmid DNA using selective precipitation by compaction agents Jason C. Murphy, Jamie A. Wibbenmeyer, George E. Fox & Richard C. Willson ...

DNA isolation and analysis week 2: Restriction enzyme digestion and electrophoretic separation Introduction Once pure samples of DNA have been prepared, it can be manipulated in several ways; cut at ...

Columns A or B were used and regenerated for 20 cycles of plasmid DNA isolation. Regenerated columns are nucleic acid-free. (b,c) Columns C and D were used for the isolation of plasmid X.

EcoRI endonuclease-generated DNA fragments carrying replication regions of the F lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ...

Electrophoretic analysis of EcoRI and HindIII restriction fragments of 2-μ m supercoiled DNA of Saccharomyces cerevisiae indicated that this class of DNA is heterogeneous and probably consists of two ...

2. Purification of DNA from cell extract In addition to DNA the cell extract will contain significant quantities of protein and RNA. A variety of procedures can be used to remove these contaminants, ...