Here we analyze the role of SR proteins in splicing of U12-type introns and in the second step of U2-type intron splicing. We show that RS domains contact the branchpoint and 5′ splice site of a ...

This editing process is called splicing, which involves removing the introns, leaving only the yellow, protein-coding regions, called exons. RNA splicing begins with assembly of helper proteins at the ...

Finally, biochemical analyses and splicing assays with various mutant SPF45 proteins helped establish the model of SPF45-dependent splicing on a short intron with a truncated PPT (Figure 1).

Group II intron RNAs self-splice in vitro but only at high salt and/or Mg²⁺ concentrations and have been thought to require proteins to stabilize their active structure for efficient splicing in vivo.

The team ultimately tested more than 32,000 5' splice sites in one intron, for every gene. Certain sequences correlated with changes in splicing efficiency, some increasing and some decreasing it.

While the mechanisms behind regulation of intron branchpoint selection are incompletely understood, Pineda and Bradley suggest that even slight alterations in these processes could result in cancerous ...

In one intron of each of the three genes, the team tested over 32,000 5' splice sites. They found that specific DNA sequences corresponded with similar splicing efficiency or inefficiency in ...

RNA splicing is a cellular process that is critical for gene expression. After genes are copied from DNA into messenger RNA, portions of the RNA that don't code for proteins, called introns, are ...

Visualizing the machinery of mRNA splicing Date: April 8, 2008 Source: Yale University Summary: Recent research at Yale provided a glimpse of the ancient mechanism that helped diversify our ...

We determined two cryo-EM structures at 3.6-Å resolution of a group II intron reverse splicing into DNA,” the investigators wrote. Source: Cell ...

Alternative splicing promotes adaptation to environmental changes; in fungi, the most common example of alternative splicing is intron retention. To explore the relationship between splicing and ...