This DNA-histone complex, called a nucleosome, poses a unique challenge both for the cell and for scientists investigating the molecular details of eukaryotic replication.

The principles uncovered in simpler organisms extend uniformly to eukaryotic organisms. First, DNA replication occurs only in one direction (5’ to 3’).

More information: Bruce Stillman et al, Mechanisms for licensing origins of DNA replication in eukaryotic cells, Nature Structural & Molecular Biology (2025). DOI: 10.1038/s41594-025-01587-5 ...

Our lab studies the mechanism of eukaryotic chromosome replication. Chromosomes are the carriers of the genetic and epigenetic information and faithful chromosome replication is of fundamental ...

A large multi-subunit protein complex, called replisome, coordinates DNA unwinding with DNA synthesis at the fork. Over 20 distinct proteins and protein complexes, comprising over 50 gene products, ...

The six-subunit complex was discovered in yeast as essential to begin DNA replication. However, a variety of experiments, particularly in lower eukaryotic cells like yeast, suggested a broader role ...

Given their roles in DNA replication and repair, it is unsurprising that scientists have targeted both PARP and PARG for anticancer efforts. 2,4 Here, PARylation is an indicator of approach efficacy, ...

From genetic experiments, we conclude that expression of the segment of yeast DNA results in the production of a diffusible substance and that transcription necessary for the complementation is most ...